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Cell Counting Kit-8（CCK-8试剂盒）
产品名称：Cell Counting Kit-8（CCK-8试剂盒）

产品货号：CK-04
规格包装：500T
品牌产地：日本同仁
产品价格：850

Cell Counting Kit-8（CCK-8试剂盒）

 

Cell Counting Kit-8（CCK-8试剂盒）是由日本同仁化学研究所（Dojindo）开发的检测细胞增殖、细胞毒性的试剂盒，为MTT法的替代方法，试剂盒中采用自己开发的水溶性四唑盐-WST-8，在日本，美国，欧洲等国家地区均持有专利，同仁化学研究所于2006年在中国获得了WST-8的注册商标。
 
CCK-8法与普通的MTT法相比，具有显著特点：
★灵敏度高，数据可靠，重现性好
★操作简便，省时省力
★水溶性，不需要换液，尤其适合于悬浮细胞
★无需放射性同位素和有机溶剂，对细胞毒性低
★为1瓶溶液，毋需预制，即开即用
★适合于高通量药物筛选
CCK-8用途：药物筛选、细胞增殖测定、细胞毒性测定、肿瘤药敏试验
CCK-8试剂盒在国内知名科研院所、重点大学、三甲医院被广泛应用，国际认可度高，使用CCK-8发表的中外文献达600多篇，其中截止08年底SCI影响因子IF＞10分的论文有37篇，最高IF26.5分
[Heterozygosity with respect to Zfp148 causes complete loss of fetal germ cells during mouse embryogenesis,Nature Genetics 33, 172 - 176 (01 Feb 2003)]
 CCK-8试剂与MTT等方法灵敏度比较
 CCK-8试剂毒性：CCK-8和其他检测方法对比可以得出——CCK-8对细胞几乎无毒性，不伤害细胞，细胞可重复利用
Cell Counting Kit-8
I公司产品
 （操作说明请参考中英文说明书或来电/在线咨询）
 
相关资料
 
CCK-8 试剂盒利用了日本同仁化学研究所（Dojindo）开发的四唑盐——WST-8 (2-(2-甲氧基-4-硝苯基)-3-(4-硝苯基)-5-(2,4-二磺基苯)-2H-四唑单钠盐)，它在电子载体1-Methoxy PMS存在的情况下能够被还原成水溶性的甲臜染料。CCK-8溶液可以直接加入到细胞样品中；不需要预配各种成分。CCK-8法是用于测定细胞增殖或细胞毒性试验中活细胞数目的一种高灵敏度，无放射性的比色检测法。CCK-8被细胞内脱氢酶生物还原后生成的橙色甲臜染料能够溶解在组织培养基中，生成的甲臜量与活细胞数量成正比。WST-8法检测细胞增殖、细胞毒性实验的灵敏度比其它四唑盐如MTT, XTT, MTS或WST-1高。由于CCK-8溶液相当稳定，并且对细胞没有毒性，因此可以长时间孵育。
 
CCK-8 检测步骤
1. 向各孔中加入100 μl细胞悬液。a)
2. 在37℃下预孵育培养板。b)
3. 向各孔中加入各浓度的待测溶液c)10 μl。
4. 37℃下孵育。
5. 向各孔中加入10 μl的CCK-8溶液d)。
6. 37℃下孵育1-4小时。e)
7. 测定450 nm处的OD值。
 
a) 使用适当的培养基制备50,000-100,000个/ml的细胞悬液。
b) 建议使用CO2培养箱过夜预培养。
c) 使用培养基或PBS来制备溶液。
d) 如果待测溶液有还原性，测定不含细胞，但含有CCK-8的待测溶液在450 nm处的空白吸光度。如果该吸光度很小，则可以直接加入CCK-8 ，如果吸光度相对较大，则需要除去培养基，并用培养基洗涤细胞两次，然后加入新的100 μl培养基和10 μl CCK-8进行检测。
e) 白细胞可能需要培养较长时间。
 
活力计算
细胞活力*（％）=[A(加药)-A（空白）]/[A（0加药）-A（空白）] ×100
A（加药）：具有细胞、CCK-8溶液和药物溶液的孔的吸光度
A（空白）：具有培养基和CCK-8溶液而没有细胞的孔的吸光度
A（0加药）：具有细胞、CCK-8溶液而没有药物溶液的孔的吸光度
*细胞活力：细胞增殖活力或细胞毒性活力
 
使用帮助
 

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