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Antibody Target: Caspase-9

Alternative Name: CASP-9 , EC 3.4.22.62 , ICE-like apoptotic protease 6 ,ICE-LAP6 ,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Immunogen: Synthetic peptide surrounding amino acid 290 of human caspase-9

Accession#: RP11-265F14.3

Gene ID: 842

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-9 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Species Reactivity: human, mouse, rat, hamster, bovine, rabbit, pig

Application: Western blot

Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects proform (~46 kDa), intermediate (~33-36 kDa), and the fully cleaved (~20-25 kDa) caspase-9.

Storage Temp.: -20 °C

Shipping: gel pack

Background Descriptions: Caspase-9 is one of the most important caspases among the caspase family members. Caspase-9 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis. The processed caspase-9 consists of large (32-35 kD) and small (10 kD) subunits which associate to form an active enzyme. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis.

Handling: The antibody solution should be gently mixed before use.

Usage: For Research Use Only! Not to be used in humans.

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