IBA1 Rat mAb

Catalog No: OB-MRT012

RRID: AB_3677303

Antigen Retrieval: For an alternative approach, tissue sections could be incubated in 1x Citrate antigen retrieval solution for 30 min at 85~95°C and cool down to room temperature before next procedure.

Immunogen: Mouse IBA1 peptide fused to GST.

Background: IBA1 (Ionized calcium binding adaptor molecule 1) is a 17-kDa EF-hand calcium-binding protein whose expression is restricted to microglia in the CNS and macrophages [1]. Expression of IBA1 is obvious in resting microglia and up-regulated in activated microglia following facialnerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia [2-5]. IBA1 functions as an actin-cross-linking protein in macrophages and microglia [6,7]. Mice homozygous for a knock-out allele of Iba1 exhibit decreased spleen weight, decreased platalet cell number and decreased susceptibility to induced arthritis with normal post-natal survival [8,9]

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[4] v Eitzen U, et al. 1998; DIO: 10.1097/00005072-199803000-00006

[5] Mori I, et al. Microbiol Immunol. 2000; DIO: 10.1111/j.1348-0421.2000.tb02556.x

[6] Sasaki Y, et al. Biochem Biophys Res Commun. 2001; DIO: 10.1006/bbrc.2001.5388

[7] Ohsawa K, et al. J Neurochem. 2004; DIO: 10.1046/j.1471-4159.2003.02213.x

[8] Casimiro I, et al. Genesis. 2013; DIO: 10.1002/dvg.22424

[9] Kishikawa S, et al. Nat Commun. 2017; DIO: 10.1038/ncomms14509