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IBA1 Guinea pig pAb
Catalog No: OB-PGP049RRID: AB_2934253Host Species: Guinea pig antibodiesImportant Notice: Antigen retrieval is recommended before immunostaining.Gene Synonyms: Aif1, allograft inflammatory factor 1, IRT-1, IRT1, Iba1Species Reactivity: Mouse, rat, human, dogApplications: IF/IHC-Fr: 1:500~2000 IF/IHC-P: 1:500~1000
Antigen Retrieval: For an alternative approach, tissue sections could be incubated in 1x Citrate antigen retrieval solution for 30 min at 95°C and cool down to room temperature before next procedure.
Immunogen: Mouse IBA1 peptide fused to GST.Purification: Affinity purificationGeneral Notes: FOR RESEARCH USE ONLYStorage Buffer: pH7.4, PBS, 55mM Tris, 50mM Glycine, ~40% Glycerol, 0.1% potassium sorbateStorage: Store at -20°C for months and -80°C for years. Avoid freeze/thaw cycle.
Background: IBA1 (Ionized calcium binding adaptor molecule 1) is a 17-kDa EF-hand calcium-binding protein which labels both macrophages and microglia [1]. In the CNS, IBA1 is specifically expressed in resting microglia and up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby has been implicated in the activation of microglia [2-5]. Iba1 functions as an actin-cross-linking protein in macrophages and microglia [6,7]. Knock-out mice exhibit decreased spleen weight and platelet cell number, and reduced susceptibility to induced arthritis with normal post-natal survival [8,9].[1] Imai Y, et al. Biochem Biophys Res Commun. 1996; DIO: 10.1006/bbrc.1996.1112[2] Ito D, et al. Brain Res Mol Brain Res. 1998; DIO: 10.1016/S0169-328X(98)00040-0[3] Ito D, et al. Stroke. 2001; DIO: 10.1161/01.str.32.5.1208[4] v Eitzen U, et al. 1998; DIO: 10.1097/00005072-199803000-00006[5] Mori I, et al. Microbiol Immunol. 2000; DIO: 10.1111/j.1348-0421.2000.tb02556.x[6] Sasaki Y, et al. Biochem Biophys Res Commun. 2001; DIO: 10.1006/bbrc.2001.5388[7] Ohsawa K, et al. J Neurochem. 2004; DIO: 10.1046/j.1471-4159.2003.02213.x[8] Casimiro I, et al. Genesis. 2013; DIO: 10.1002/dvg.22424[9] Kishikawa S, et al. Nat Commun. 2017; DIO: 10.1038/ncomms14509
Key Words: microglia, macrophages, regeneration, injuries, immunology, CNS, immune system, monocyte
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