Hoechst 33342 (ultra pure)
产品名称:Hoechst 33342 (ultra pure)


Hoechst 33342 (ultra pure)

DNA dye
ENZ-52401 100 mg   CNY:1653    
Hoechst 33342 is fluorescent probe useful for detecting DNA by fluorescence microscopy and flow cytometry. Hoechst 33342 may be used on live or fixed cells and is also applicable for cell cycle analysis and monitoring DNA condensation by flow cytometry. An additional ethyl group renders Hoechst 33342 more lipophilic, and thus more readily cell permeable than Hoechst 33258 . Exclusion of Hoechst 33342 dye is a characteristic common to stem cells, as well as chemotherapy-resistant cancer cells. Wavelength Maxima: Excitation 350nm, Emission 461nm

Product Specification

Alternative Name: 2’-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole
Formula: C27H37Cl3N6O4
MW: 615.9 (trihydrochloride, trihydrate)
CAS: 23491-52-3
Purity: ≥95% (HPLC)
Appearance: Green solid.
Solubility: Soluble in water (50mg/ml) or DMSO (10mg/ml).
Long Term Storage: -20°C
Use/Stability: Stable for at least one year after receipt when stored as recommended.
Handling: Protect from light. Keep cool and dry.
Background / Technical Information: This product is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLestial® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required.
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Product Literature References

Hormone-induced 14-3-3γ adaptor protein regulates steroidogenic acute regulatory protein activity and steroid biosynthesis in MA-10 Leydig cells: Y. Aghazadeh et.al; J. Biol. Chem. 15380 (2012), Application(s): activation of cAMP-dependent protein kinase (PKA)., Abstract; Full Text
Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc. 2, 187 (2007), Abstract;
High-throughput microtiter assay for Hoechst 33342 dye uptake: G.M. Seigel & L.M. Campbell; Cytotechnology 45, 155 (2004), Abstract;